Binding assay system and method of making and using same

Details Binding assay system and method of making and using same Binding assay system and method of making and using same

G - Physics

01

N

167/44, 150/9

G01N 33/543 (2006.01) B01L 3/00 (2006.01) G01N 35/10 (2006.01)

Patent

CA 1231893

ABSTRACT OF THE DISCLOSURE Method and apparatus for use in the diag- nostic analysis of a liquid specimen through binding assays. The apparatus includes a rigid body having an elongated, shallow well formed in it, with a plurality of elongated strips, preferably in the form of cotton threads, stretched across the well in spaced relationship and generally perpendicular to the well's longitudinal axis. Each thread is coated with a separate binding conjugate such as an antigen or allergen. A cover plate that includes a thin plastic layer and an overlaying thin metallic layer covers the well to form an enclosed chamber having a specific volume. The metallic layer includes a separate elongated aperture in alignment with each coated thread. A liquid specimen is introduced into the chamber through one of two ports, for incubation with the coated threads, after which the specimen is removed and the reaction that occurred on each thread is deter- mined.

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