Binding assay using a solid ion exchange adsorbent

Details Binding assay using a solid ion exchange adsorbent Binding assay using a solid ion exchange adsorbent

G - Physics

01

N

167/41

G01N 23/00 (2006.01) G01N 33/538 (2006.01)

Patent

CA 1108530

ABSTRACT OF THE DISCLOSURE A method and test means for determining a specific bind- ing substance, such as an antigen or hapten, in a liquid sample wherein a solid, nonspecific adsorbent is contacted, preferably in sequence, with the sample and a labeled form of a binding partner for the substance to be determined, such as a labeled antibody. The amount of label which becomes associated with the adsorbent by binding of the labeled bind- ing partner to the adsorbed substance to be determined, i.e., in a sandwich fashion, is a function of the amount of the sub- stance to be determined in the liquid sample. Preferably, the adsorbent is an ion exchange material, and the assay steps are carried out under pH conditions between the isoelectric points (pI values) of the substance to be determined and the labeled binding partner. The method and test means are particularly suited for the detection of hepatitis B surface (HBs) antigen, especially where the label is a radioactive material and a column format is employed. The resultant radioimmunoadsorbent (RI-AdTM) assay and test means for HBS antigen offers signifi- cant manufacturing and user advantages over the prior art methods and test means.

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