Bio-chip, photoluminescent methods for identifying...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2424722

A method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for its single stranded nucleic acid sequence to bind to a material of interest, photoluminescence from the sensor can be measured. An apparatus for tagging-free detection of binding of molecules also is provided. Methods of making tagging-free sensors are provided. Also, tagging-free methods to detect binding of antigens and related devices are disclosed.

L'invention concerne une méthode permettant de détecter avantageusement une liaison de molécules sans marquer les molécules d'un échantillon. On utilise un capteur comprenant une séquence d'acide nucléique à un seul brin et un matériau photoluminescent en couches respectives. Une fois qu'on a exposé ledit capteur à un échantillon biologique pendant une durée suffisante pour lier la séquence d'acide nucléique à un seul brin à un matériau considéré, il est possible de mesurer la photoluminescence à partir de ce capteur. L'invention concerne également un appareil permettant de détecter une liaison de molécules sans avoir recours à un marquage. L'invention concerne enfin des méthodes permettant de fabriquer des capteurs sans marquage ainsi que des méthodes n'ayant pas recours au marquage pour détecter une liaison d'antigènes, et des dispositifs associés.

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