Bio-reactor for tissue cultivated in the form of a thin...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/04 (2006.01)

Patent

CA 2439731

The invention relates to a cell and tissue culture reactor which is particularly suitable for culturing cells or tissues in the form of a thin layer, and which comprises means enabling it to ensure physiological stimulation and nutrition in a culture medium adapted to the reduced thickness of the culture. The reactor can be used in the preparation of an implant and also comprises means for ensuring mechanical stimulation of tissues. The reactor further comprises means or has a shape making it easier to introduce the tissue or the solid part of an implant into a culture chamber, and for example, a wall or an element which is elastically deformable. The reactor can also comprise means for regulating the temperature. The reactor can be used to culture different types of cells and in the preparation of implants having various forms, compositions and uses.

L'invention concerne un réacteur de culture cellulaire et tissulaire particulièrement adapté à la culture de cellules ou tissus de structure en couche mince, et comprend des moyens permettant d'assurer une stimulation physiologique et une alimentation en milieu de culture adaptée à l'épaisseur réduite de la culture. Le réacteur peut servir à la préparation d'un implant et comporte également des moyens permettant d'assurer une stimulation mécanique des tissus ou cellules et une alimentation en milieu de culture adaptée aux tissus de structure. Le réacteur comporte également, de manière avantageuse, des moyens ou une forme permettant de faciliter l'introduction du tissu ou de la partie solide d'un implant dans la chambre de culture, et par exemple une paroi ou un élément déformable élastiquement. Le réacteur peut en outre comporter des moyens de régulation de la température. Le réacteur est utilisable pour la culture de différents types cellulaires et la préparation d'implants de formes, compositions et applications variées.

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