Bioactive purified hspe7 compositions

A - Human Necessities – 61 – K

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Details

A61K 39/39 (2006.01) A61K 39/12 (2006.01) A61P 31/20 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2653474

A method of increasing the biological activity of a purified Hsp65 - E7 fusion protein (HspE7) is provided. The method comprises admixing the HspE7 along with an immune stimulant selected from the group consisting of CpG, a TLR3 agonist such as PoIyLC, PoIyICLC, mono-phosphoryl-lipid A (MPL), MPL- trehalose 6,6'-dimycolate (MPL-TDM), and anti-CD40. A composition comprising HspE7 and one or more than one of CpG, a TLR3 agonist such as PoIyLC, PoIyICLC, MPL, MPL-TDM, and anti-CD40, and method of reducing a tumor or virus development in a mammal or subject in need thereof by using the composition are also provided.

La présente invention concerne un procédé permettant d'augmenter l'activité biologique d'une protéine de fusion Hsp65 - E7 (HspE7) purifiée. Le procédé permet de mélanger la HspE7 avec un stimulant immun choisi dans le groupe constitué par le CpG, un agoniste de TLR3 tel que PoIyLC, PoIyICLC, un lipide A monophosphorylé (MPL), du MPL-tréhalose-6,6'-dimycolate (MPL-TDM), et un anti-CD40. L'invention concerne également une composition contenant la HspE7 et un ou plusieurs des composés parmi le CpG, un agoniste de TLR3 tel que PoIyLC, PoIyICLC, le MPL, le MPL-TDM, et un anti-CD40, ainsi qu'un procédé permettant de réduire une tumeur ou le développement d'un virus chez un mammifère ou chez un sujet nécessitant des soins à l'aide de la composition.

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