Bioassay for detecting...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/10 (2006.01) C12Q 1/02 (2006.01) C12Q 1/66 (2006.01) G01N 33/50 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2200678

A recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL--12286, also known as the mouse H1L1.1 cell line, has been made by using genetic engineering techniques for inserting dioxin responsive elements upstream of a luciferase reporter gene, and then, transfecting the resultant recombinant expression plasmid, identified as pGudLuc1.1, into mouse hepatoma cells. The mouse H1L1.1 cell line is useful in a method for the quantitative analysis of polyaromatic hydrocarbons in samples.

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