Biochemical analysis of partitioned cells

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2653321

The invention relates to compositions and methods for the analysis of biomolecules associated with cells, where the presence or absence of a particular biomolecule (e.g., an expressed protein or a nucleic acid gene expression product) associated with the cells is examined. The invention provides methods for single cell biochemical analysis, as well as instrumentation for the single-cell biochemical analysis. Most advantageously, the invention affords methods and instrumentation for high-throughput biochemical analysis of large numbers of single cells.

L'invention concerne des compositions et des procédés destinés à l'analyse de biomolécules associées à des cellules, la présence ou l'absence d'une biomolécule particulière (par exemple une protéine exprimée ou le produit d'expression génique d'un acide nucléique) associée aux cellules étant examinée. L'invention concerne des procédés destinés à une analyse biochimique de cellules isolées aussi bien que l'instrumentation nécessaire à l'analyse biochimique de ces cellules isolées. Selon le mode de réalisation le plus avantageux, l'invention concerne des procédés et une instrumentation permettant une analyse biochimique à haut rendement de grands nombres de cellules isolées.

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