Biochip

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2510719

The invention relates to a biochip comprising a flat carrier and an array of spots containing catcher molecules which are arranged on said carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the catcher molecules and the target molecules applied by means of an analyte solution. In order to increase the sensitivity or the binding-specific measuring effects of the bio-chip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing catcher molecules and which is permeable to target molecules.

L'invention concerne une biopuce qui comporte un support plat ainsi qu'un réseau de spots contenant des molécules pièges, placé sur ce dernier. Un réseau de microélectrodes, servant à la détection, par spectroscopie d'impédance, d'événements de liaison entre les molécules pièges et des molécules cibles appliquées au moyen d'une solution d'analyte, est associé à chaque spot. L'objectif de l'invention est d'accroître la sensibilité ou l'effet de mesure, spécifique des liaisons, de la biopuce. A cet effet, le réseau d'électrodes est incorporé au moins partiellement dans une couche de réaction hydrophile contenant des molécules pièges et perméable aux molécules cibles.

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