Biochip and the production method thereof

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2453103

The invention relates to a biochip comprising a substrate having a main reflective surface. The inventive biochip is characterised in that the main surface is provided with areas that are made sensitive to fluorescence detection by a transparent layer of thickness (2k+1).lambda./4, wherein k is a positive integer or zero, .lambda. denotes a wavelength that is included between a fluorescence excitation wavelength .lambda.o and a fluorescence emission wavelength .lambda.1. Said biochip can be produced by depositing a transparent layer of thickness m.lambda.'/2 on a substrate and creating pits or bumps of thickness (2k+1).lambda./4 in said layer in order to form sensitive areas.

L'Invention est relative à une biopuce comprenant un substrat présentant une surface principale réfléchissante caractérisée en ce que la surface principale présente des sites rendus sensibles à une détection de fluorescence par une couche transparente d'épaisseur (2k+1) lambda/4, avec k entier positif ou nul, lambda désignant une longueur d'onde comprise entre une longueur d'onde lambda o d'excitation de fluorescence, et une longueur d'onde lambda 1 d'émission de fluorescence. Elle peut être fabriquée en déposant sur un substrat une couche transparente d'épaisseur m lambda'/2 et en réalisant dans cette couche des puits ou des plots d'épaisseur (2k+1) lambda/4 pour former les sites sensibles.

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