Biochips including ion transport detecting structures and...

G - Physics – 01 – N

Patent

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G01N 27/26 (2006.01) B01D 57/02 (2006.01) B01D 59/42 (2006.01) B01D 59/50 (2006.01) B01D 61/42 (2006.01) B01D 61/58 (2006.01) B01L 3/00 (2006.01) B03C 5/02 (2006.01) C02F 1/469 (2006.01) C07K 1/26 (2006.01) C08F 2/58 (2006.01) C12Q 1/68 (2006.01) C25B 7/00 (2006.01) C25B 15/00 (2006.01) G01F 1/64 (2006.01) G01L 1/20 (2006.01) G01L 9/18 (2006.01) G01N 15/10 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01) B81B 1/00 (2006.01)

Patent

CA 2441366

The present invention recognizes that the determination of ion transport function or property using direct detection methods, such as patch-clamps, whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and methods of use that allow for the direct analysis of ion transport function or property using microfabricated structures that can allow for automated detection of ion transport function or property. These biochips and methods of use thereof are particularly appropriate for automating the detection of ion transport function or property, particularly for screening purposes.

L'invention permet de déterminer la fonction ou la propriété de transport d'ions au moyen de procédés de détection directs, tels que des dispositifs de type patch-clamp, d'enregistrement de cellule entière ou d'enregistrement de canal unique, lesquels sont préférables à ceux utilisant des procédés de détection indirects, tels qu'un système de détection fondé sur le principe du transfert d'énergie par résonance de fluorescence (FRET). Cette invention concerne des biopuces et des procédés d'utilisation permettant l'analyse directe de la fonction ou de la propriété de transport d'ions au moyen de structures microfabriquées permettant la détection automatisée de fonction ou de propriété de transport d'ions. Ces biopuces et les procédés d'utilisation de celles-ci conviennent particulièrement à la détection automatisée de la fonction ou de la propriété de transport d'ions, notamment à des fins de criblage.

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