Biocidal chitin binding proteins

Details Biocidal chitin binding proteins Biocidal chitin binding proteins

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01N 37/18 (2006.01) A01N 43/50 (2006.01) A01N 65/00 (2006.01) A61K 38/16 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2147122

Biocidal proteins capable of isolation from seeds have been characterised. The proteins have an amino acid sequence con- taining the common cysteine/glycine domain of Chitin-binding Plant Proteins but show substantially better activity against pa- thogenic fungi, a higher ratio of basic amino acids to acidic amino acids, and/or antifungal activity which results in increased hy- phal branching. Antimicrobial proteins isolated from Capsicum, Briza and related species are provided. The proteins show a wide range of antifungal activity and are active against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants may be transformed with this DNA. The proteins find agricultural or pharmaceutical applica- tion as antifungal or antibacterial agents. Transgenic plants expressing the protein will show increased disease resistance.

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