Biocontrol agents for control of root diseases

A - Human Necessities – 01 – N

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A01N 63/00 (2006.01) C12N 1/20 (2006.01) C12N 15/52 (2006.01) C12N 15/78 (2006.01)

Patent

CA 2309884

Fluorescent Pseudomonas spp. are described which are effective for the control of diseases caused by one or more of Rhizoctonia, Gaeumannomyces graminis and Pythium. The subject biocontrol strains have a unique genotype as shown by a characteristic banding pattern, and exhibit root-colonizing ability which is characterized by both higher population density on roots and extended colonizing activity compared to known Gg-suppressive strains. A further property is the ability of a strain to duplicate the level of biocontrol obtained naturally in a take-all decline soil. Methods for isolation and identification of the strains and their use to control diseases caused by Gg are provided. Transgenic fluorescent Pseudomonas spp. which have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol and have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome are also described.

Cette invention porte sur des souches de l'espèce Pseudomonas fluorescens efficaces dans la lutte contre des maladies causées par l'un de ces végétaux, Rhizoctonia, Gaeumannomyces graminis et Pythium, sinon par plusieurs d'entre eux. Les souches de lutte biologique présentées dans cette invention, qui possèdent un génotype unique comme le démontre une configuration spécifique de banding, font preuve d'une aptitude à coloniser les racines caractérisée par une densité de population et une activité colonisatrice supérieures à celle des souches connues agissant comme suppresseur de Gaeumannomyces graminis (Gg). Une autre propriété de cette souche est sa capacité à multiplier par deux le niveau de lutte biologique naturellement obtenu dans le sol avec la régression du piétin-échaudage du gazon. L'invention porte également sur des techniques d'isolation et d'identification de ces souches ainsi que sur leur utilisation dans la lutte contre des maladies causées par Gg. Cette invention concerne, de surcroît, des Pseudomonas fluorescens transgéniques possédant, d'une part, un locus codant la production de l'antibiotique 2,4-diacétyl-phloroglucine et, d'autre part, un locus biosynthétique, introduit de manière stable dans leur génome, codant la production d'un autre antibiotique, en l'occurrence, l'acide carboxylique-1-phénazine.

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