Bioconversions catalyzed by the toluene monooxygenase of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/52 (2006.01) C12P 1/00 (2006.01) C12P 7/42 (2006.01) C12P 7/54 (2006.01) C12P 17/10 (2006.01)

Patent

CA 2069742

2069742 9206208 PCTABS00011 Disclosed and claimed are toluene monooxygenase (TMO) gene sequences from Pseudomonas mendocina KR-1, TMO proteins encoded by these sequences, recombinant plasmids containing such sequences, and microorganism host cells containing such plasmids. A five-gene and six-gene TMO gene cluster encode proteins that are useful in a variety of bioconversions. In particular, the TMO gene cluster is useful for the preparation of p-hydroxyphenylacetic acid and indigo. In addition, the TMO gene cluster is useful for the degradative bioconversion of toxic compounds, such as trichloroethylene.

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