Biological and analytical uses of phenalenone and...

C - Chemistry – Metallurgy – 12 – Q

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150/9, 150/16.1

C12Q 1/02 (2006.01) C07C 271/50 (2006.01) C07C 271/54 (2006.01) C12Q 1/04 (2006.01) G01N 33/52 (2006.01) G01N 33/58 (2006.01) G01N 33/04 (2006.01)

Patent

CA 1284932

BIOLOGICAL AND ANALYTICAL USES OF PHENALENONE AND BENZPHENALENONE COMPOUNDS Abstract of the Disclosure Certain phenalenone and benzphenalenone fluorescent compounds are useful in biomedical studies and analytical determinations. These com- pounds are particularly useful in assays for living organisms, e.g. microorganisms, carried out at a pH of 9 or less. For these determinations, the com- pounds can be attached to reducible compounds which will subsequently release the fluorescent moiety upon reduction. In the presence of an electron transfer agent, the reducible compounds are easily reduced by a microorganism.

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