Biological assay for microbial contamination

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) C12Q 1/06 (2006.01) C12Q 1/22 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2190516

Methods and kits for detecting the presence of and quantifying the concentration of viable microorganisms in a liquid sample are provided. Enzymes present in viable microorganisms reduce an indicator reagent to produce a visible color change. No titrations or dilutions of the sample are necessary. Variations of the test are described which can be used to detect a threshold level of viable microorganisms, to detect the presence or absence of viable microorganisms and to quantify the concentration of viable microorganisms in a liquid sample. The results are obtained rapidly with those for the detection available within thirty minutes and those for the quantification available within thirteen hours.

L'invention concerne des procédés et des matériels destinés à détecter la présence et à quantifier la concentration de microorganismes viables dans un échantillon liquide. Des enzymes présentes dans des micro-organismes viables réduisent un réactif indicateur afin de produire un changement de couleur visible. Aucun titrage ni aucune dilution de l'échantillon n'est nécessaire. On peut utiliser des variantes du test afin de détecter un niveau seuil de micro-organismes viables, afin de détecter la présence ou l'absence de micro-organismes viables et afin de quantifier la concentration de ces derniers dans un échantillon liquide. On obtient les résultats rapidement, ceux de la détection étant disponibles en l'espace de 30 minutes et ceux de la quantification en l'espace de 13 heures.

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