Biological extracts and method for making same

Details Biological extracts and method for making same Biological extracts and method for making same

C - Chemistry, Metallurgy

12

N

195/33.1

C12N 5/00 (2006.01) C12N 9/99 (2006.01) G01N 33/579 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1153714

ABSTRACT OF THE DISCLOSURE Abstract of the Disclosure Endotoxin clottable extracts are standardized by inactivating endogenous inhibitor, determining the coagulase activity of the extract and proportioning the extract into an endotoxin assay reaction mixture so as to provide coagulase activity at a predetermined level. In most circumstances the coagulogen concentration is also determined and, if the concentration is lower than a predetermined amount, coagulogen is added to the extract. Inhibitor is advantageously inactivated by adjusting the extract pH to a strongly alkaline level, incubating and readjusting the pH to near neutrality.

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