C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 33/06 (2006.01)
Patent
CA 2258282
A biological process for producing steroids hydroxylated at the 25-position thereof comprises adding steroids (excluding cholesterol) to the cells or culture liquid of a microorganism of the genus Amycolata or Sphingomonas capable of hydroxylating the steroids at the 25--position thereof to convert a hydrogen atom bonded to a carbon atom at the 25-position of each steroid into hydroxyl group. By this process, steroids(other than cholesterol) can be biologically hydroxylated at the 25-position by using microorganisms other than those of the genus Streptomyces.
Procédé biologique servant à préparer des stéroïdes 25-hydroxylés et consistant à ajouter un stéroïde (à l'exception de cholestérol) à des cellules microbiennes d'un amycolata ou sphingomonas capable d'hydroxyler les stéroïdes, ou une de leurs cultures, au niveau de la position 25, de manière à remplacer l'atome d'hydrogène relié à l'atome de carbone en position 25 du stéroïde par un groupe hydroxyle. Ce procédé permet d'hydroxyler biologiquement tous les stéroïdes, à l'exception de cholestérol, au niveau de la position 25 au moyen d'un micro-organisme différent de ceux appartenant au groupe Streptomyces.
Dobashi Kazuyuki
Takeda Koji
Terasawa Tadashi
Yoshioka Takeo
Chugai Seiyaku Kabushiki Kaisha
Dennison Associates
Mercian Corporation
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