A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 35/12 (2006.01) A61K 35/407 (2006.01) C12M 3/00 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2244659
The invention relates to a solid support for use in cell cultivation in vitro, comprising a 3D-matrix material and hollow fibres being permeable to at least gaseous oxygen and/or gaseous carbon dioxide, a biological reactor for the cultivation and/or maintenance of living cells comprising said solid support, as well as a method for culturing and/or maintaining living cells, using said solid support and/or said biological reactor. The solid support, biological reactor and method are especially suited for culturing human or animal derived liver cells, for use in or as a bio-artificial liver. The invention further relates to such a bio-artificial liver, as well as a method for treating liver disorders using said bio-artificial liver.
L'invention porte sur un support solide de culture de cellules in vitro comportant un matériau matrice 3D, des fibres creuses perméables au moins à l'oxygène gazeux et/ou au CO2, un réacteur biologique pour la culture et/ou le maintien en vie de cellules vivantes comprenant ledit support solide. Elle porte en outre sur un procédé de culture et/ou de maintien en vie de cellules vivantes à l'aide dudit support et/ou dudit réacteur biologique. Le support, le réacteur et la méthode sont particulièrement adaptés à la cultures de cellules hépatiques humaines ou animales pouvant constituer partiellement ou totalement des foies bio-artificiels. L'invention porte en outre sur de tels foies artificiels et sur une méthode de traitement des troubles hépatiques les utilisant.
Academisch Ziekenhuis Bij de Universiteit Van Amsterdam
Mccarthy Tetrault Llp
Seed Capital Investments-2 (sci-2) B.v.
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