Biological specimen collection/transport compositions and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2759028

Disclosed are compositions for collecting, storing, and transporting populations of nucleic acids from biological specimens, and clinical, forensic, or environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. In particular embodiments, the invention provides a single, one-step, sample collection/transport/storage formulation containing a known quantity of a non-genomic, nucleic acid carrier molecule that serves as an internal reference control to monitor the fidelity of the collection/transportation medium, and measure the integrity of nucleic acids subsequently isolated and purified from the processed sample.

L'invention porte sur des compositions pour collecter, stocker et transporter des populations d'acides nucléiques provenant de spécimens biologiques et d'échantillons cliniques, de médecine légale ou environnementaux. Elle porte aussi sur des procédés d'utilisation de ces compositions en tant que formulations en une étape pour tuer les pathogènes, pour inactiver des nucléases et pour libérer des polynucléotides à partir d'autres composants cellulaires se trouvant à l'intérieur de l'échantillon, et pour stabiliser les acides nucléiques avant traitement ou analyse plus poussée. Dans des formes de réalisation particulières, l'invention porte sur une formulation unique, en une étape, de collecte/transport/stockage d'échantillons, contenant une quantité connue d'une molécule support d'acide nucléique non génomique qui sert de témoin de référence interne pour surveiller la fidélité du milieu de collecte/transport, et mesurer l'intégrité des acides nucléiques ultérieurement isolés et purifiés à partir de l'échantillon traité.

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