Biological systems for manufacture of polyhydroxylalkanoate...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12N 15/70 (2006.01) C12N 15/82 (2006.01) C12P 7/62 (2006.01) C08G 63/06 (2006.01)

Patent

CA 2303070

The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtained by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, .alpha.-ketoglutarate, or succinate as substrate.

On a isolé à partir de bactéries le gène codant une transférase A 4-hydroxybutyryle-Co et on l'a intégré dans le génome de bactéries exprimant également une synthase polyhydroxyalcanoate pour créer un procédé de production amélioré de polyhydroxyalcanoate contenant 4HB à l'aide d'organismes transgéniques comprenant des bactéries et des plantes. Ces nouveaux mécanismes constituent un moyen permettant de produire des PHAs contenant 4HB à partir de sources de carbone peu coûteuses telles que des sucres et des acides gras avec des rendements élevés, qui sont stables. Des souches utiles sont obtenues par criblage de souches dans le génome desquelles sont intégrés un gène codant une transférase CoA-4HB et/ou une synthase PHA pour produire des polymères. Des procédés de production de polymère comprennent l'utilisation de systèmes de recombinaison pouvant utiliser des substrats peu coûteux. On décrit des systèmes qui peuvent utiliser les mécanismes de dégradation des acides aminés, du .alpha.-cétoglutarate ou du succinate en tant que substrat.

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