C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/22 (2006.01) C12M 1/12 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/25 (2006.01)
Patent
CA 2169546
The invention is directed to biological processes and apparatus for determining the efficacy of a sterilization cycle based upon the recovery of activity of interactive enzyme systems comprising enzymes, coenzymes, catalysts, cofactors, substrates or any other necessary reagents. The invention provides a vital process for expediting sterility verification before utilization of the articles thought to be sterilized. The invention involves the rapid detection of any surviving interactive enzymatic activity which directly relates to the probability of any biological spores surviving in a test sample. An absence of a change indicates that the sterilization process had inactivated the enzyme system thereby preventing the interactive reaction from taking place which is a rapid equivalent to directly detecting the survivability of bacterial spores in a similar test. The methods and apparatus of the invention are useful in the health care industry such as in hospitals, laboratories, and research institutions, in food and environmental technology, and in all technologies which utilize sterilization in manufacturing, production or waste disposal.
L'invention concerne des procédés biologiques ainsi qu'un appareil de détermination de l'efficacité d'un cycle de stérilisation basé sur la récupération d'activités de systèmes enzymatiques interactifs comprenant des enzymes, des coenzymes, des catalyseurs, des cofacteurs, des substrats et tout autre réactif nécessaire. L'invention a trait à un procédé vital permettant d'effectuer une vérification de stérilité avant l'utilisation des articles dont on pense qu'ils ont été stérilisés. L'invention consiste en la détection rapide de toute activité enzymatique interactive subsistante directement rapportée à la probabilité d'une subsistance d'éventuelles spores biologiques dans un échantillon test. L'absence d'un changement indique que le procédé de stérilisation a eu pour effet d'inactiver le système enzymatique, empêchant ainsi l'apparition d'une réaction interactive constituant un équivalent rapide à la détection directe de la capacité de survivance de spores bactériennes dans un test similaire. Les procédés et l'appareil de l'invention
Burnham Jeffrey C.
Hageage George J.
Hendricks Judy
Jambard-Sweet Douglas
3m Innovative Properties Company
Mbm Intellectual Property Law Llp
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