Bioluminescent immunoassays

G - Physics – 01 – N

Patent

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150/14, 150/3, 1

G01N 33/58 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1234756

ABSTRACT A process for detecting ligand-ligand interactions such as antigen-antibody, hapten-antibody, hormone-receptor, protein-nucleic acid, and nucleic acid-nucleic acid utili- zing a coelenterate-derived luciferase or a coelenterate-- derived photoprotein which is a bioluminescent, low molecu- lar weight, single polypeptide chain as a label in a bio- luminescence-linked immunoassay is disclosed. The disclosed process is rapid and process provides greater sensitivity over existing processes. Also, the disclosed process uti- lizes labels and reagents which possess a shelf life of years. Three types of bioluminescence-lined immunoassays are disclosed: heterogeneous assay requiring a single sepa- ration step, homogeneous assay without separation step requiring dual wavelength measurements, and a homogeneous assay without a separation step requiring a single wavelength measurement.

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