Bioluminescent methods for direct visual detection of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/63 (2006.01) C12N 15/78 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2419481

The invention relates to devices and methods that utilize immobilized bacterial bioreporters genetically engineered to emit light visible to the naked eye in the presence of selected analytes. An exemplary bioreporter is an E. coli that has been modified to respond to mercury II as a result of incorporation of a merRop/Iux gene cassette into its genome. Systems employing analagously engineered microorganisms can detect selected toxins quickly without need for expensive instruments or highly trained technicians.

L'invention concerne des dispositifs et des procédés faisant appel à des bio-détecteurs bactériens immobilisés modifiés génétiquement de manière à émettre une lumière visible à l'oeil nu en présence des analytes choisis. Un exemple de ces bio-détecteurs est une bactérie E.coli qui a été modifiée par l'incorporation dans son génome d'une cassette du gène merRop/lux de manière à réagir au mercure II. Des systèmes faisant appel à des micro-organismes produits de manière analogue permettent de détecter rapidement les toxines choisies, sans qu'il soit nécessaire de recourir à des instruments coûteux ou à des techniciens hautement spécialisés.

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