Bioluminescent plants and methods of making same

Details Bioluminescent plants and methods of making same Bioluminescent plants and methods of making same

A - Human Necessities

01

H

A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/52 (2006.01) C12N 15/82 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2442911

A genetically modified plant cell containing a heterologous nucleotide sequence encoding a bioluminescent polypeptide, which is expressed in amount sufficient to produce at least about 750,000 photons of visible light/mm2/second, is provided, as is a visibly bioluminescent transgenic plant, which contains such a genetically modified plant cell. Also provided is a recombinant nucleic acid molecule, which contains a plant translational enhancer operatively linked to a nucleotide sequence encoding a bioluminescent polypeptide. In addition, methods of producing a genetically modified plant cell that is visibly bioluminescent introducing a transgene encoding a bioluminescent polypeptide into a plant cell, whereby the bioluminescent polypeptide is expressed at a level that produces at least about 750,000 photons of visible light/mm2/second are provided, as are kits containing such visibly bioluminescent compositions.

L'invention concerne une cellule de plante génétiquement modifiée contenant une séquence nucléotidique hétérologue codant un polypeptide bioluminescent, laquelle est exprimée en quantité suffisante pour produire au moins environ 750000 photons de lumière visible/mm?2¿/seconde, ainsi qu'une plante transgénique visiblement bioluminescente, laquelle contient une telle cellule de plante génétiquement modifiée. L'invention concerne également une molécule d'acide nucléique recombinée, laquelle contient un activateur de traduction de plante lié fonctionnellement à une séquence nucléotidique codant un polypeptide bioluminescent. De plus, l'invention concerne des méthodes de production d'une cellule de plante génétiquement modifiée laquelle est visiblement bioluminescente, consistant à introduire un transgène codant un polypeptide bioluminescent dans une cellule de plante, de manière que le polypeptide bioluminescent est exprimé à un niveau produisant au moins environ 750000 photons de lumière visible/mm?2¿/seconde et des matériels contenant ces compositions visiblement bioluminescentes.

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