Bioremediation of pollutants with butane-utilizing bacteria

C - Chemistry – Metallurgy – 02 – F

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C02F 3/34 (2006.01) B01D 53/70 (2006.01) B09C 1/10 (2006.01) C02F 3/00 (2006.01) C12N 1/20 (2006.01) A62D 3/00 (2006.01)

Patent

CA 2275414

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In situ or ex situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

On utilise des bactéries consommant du butane pour dégrader des polluants hydrocarbures tels que du thrichloroéthylène (TCE). On peut employer des techniques in situ ou ex situ pour réduire ou éliminer des polluants hydrocarbures présents dans des sources liquides, gazeuses et solides. Dans une forme de réalisation préférée, on réduit les concentrations de TCE dans divers environnements aqueux au moyen d'une mise en contact d'une source d'eau contaminée avec des bactéries utilisant du butane en présence d'oxygène pour dégrader le TCE par cométabolisme ou par métabolisme direct. Les bactéries utilisat du butane déterminées appropriées sont: Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium et Cytophaga. Les bactéries utilisant du butane ont une toxicité TCE relativement faible comparativement aux bactéries utilisant du méthane et démontrent une aptitude améliorée pour dégrader le TCE.

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