Biosensor

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) G01N 33/532 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01) G01N 33/66 (2006.01)

Patent

CA 2316199

The present invention relates to a glucose biosensor comprising a genetically engineered Glucose Binding Protein (GBP). In a specific embodiment, the invention relates to a GBP engineered to include mutations that allow site specific introduction of environmentally sensitive reporter groups. The signal of these prosthetic groups changes linearly with the degree of glucose binding. Thus, the glucose sensor of the invention can be used, for example, for detection of glucose in blood or industrial fermentation processes.

La présente invention concerne un biocapteur de glucose comprenant une protéine de liaison au glucose génétiquement modifiée. Selon un mode de réalisation spécifique, l'invention concerne une protéine de liaison au glucose modifiée pour comprendre des mutations qui permettre l'introduction spécifique à un site de groupes reporters sensibles à l'environnement. Le signal de ces groupements prosthétiques change de manière linéaire en fonction du degré de liaison au glucose. Ainsi, le capteur de glucose selon l'invention peut être utilisé, par exemple, pour détecter le glucose dans le sang ou dans des processus de fermentation industriels.

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