Biosensor

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2342118

The present invention relates to a method for evaluating biological effects in a substance which method comprises sampling the substance, subjecting the sample to an assay in the presence of a biosensor engineered with a gene which expresses a light emitting protein and noting the light output; characterised in that the biosensor is eukaryotic and the substance is assayable over any pH between pH1 and pH12. Such a eukaryotic biosensor may be derived from Saccharomyces cerevisiae and is useful in assaying potable waters.

La présente invention concerne une méthode d'évaluation des effets biologiques dans une substance, laquelle méthode consiste à échantillonner la substance, à soumettre l'échantillon à un dosage en présence d'un biocapteur conçu avec un gène exprimant une protéine de photoémission et à relever la production de lumière; caractérisée en ce que le biocapteur est eucaryote et la substance peut être dosée à n'importe quel pH entre pH1 et pH12. Ce biocapteur eucaryote peut être dérivé de Saccharomyces cerevisiae et il est utile pour l'analyse des eaux potables.

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