Biosensor

G - Physics – 01 – N

Patent

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Details

G01N 27/327 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2457964

The present invention relates, in general, to biosensors and, in particular, to bioelectronic sensors comprising a macromolecule immobilized on an electrode surface so that a redox cofactor that is site-specifically attached to the surface of the macromolecule is between the macromolecule and electrode surface ligand-mediated conformational changes alter the geometry of interaction between the redox cofactor and the electrode surface resulting in a change in electronic coupling between the cofactor and electrode.

La présente invention concerne, de manière générale, des biocapteurs et notamment, des capteurs bioélectroniques comprenant une macromolécule immobilisée sur une surface d'électrode de sorte qu'un cofacteur redox, fixé de manière spécifique au site sur la surface de la macromolécule, est situé entre la macromolécule et l'électrode. Les changements conformationnels induits par le ligand de surface modifient la géométrie d'interaction entre le cofacteur redox et la surface d'électrode, entraînant un changement au niveau du couplage entre le cofacteur et l'électrode.

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