Biosensor and method of making

Details Biosensor and method of making Biosensor and method of making

G - Physics

01

N

G01N 27/403 (2006.01) B23K 26/36 (2006.01) B32B 15/00 (2006.01) G01N 27/327 (2006.01) C12Q 1/00 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 2638973

A method of manufacturing a plurality of biosensors, comprises: (a) providing a web of base substrate material having a metal conductive layer formed thereon; (b) projecting an image of an electrode pattern onto the metal conductive layer with a laser apparatus, wherein an electrode pattern that corresponds to the image is formed by laser ablation on the web of base substrate material, (c) moving one of the laser apparatus and the web of base substrate material and repeating step (b) a plurality of times to produce a plurality of the electrode patterns at spaced intervals along the web of base substrate material; (d) depositing a reagent on the web of base substrate material and at least partially covering each electrode pattern of the plurality of electrode patterns with the reagent; (e) laminating at least one web of a covering layer or a spacing layer over the web of base substrate material, thereby forming a cover and a sample-receiving chamber for each biosensor; and (f) cutting through the at least one web of the covering layer or the spacing layer and the web of base substrate material to form the plurality of biosensors. The biosensors so produced possess smooth, high-quality edges with a good edge definition. These smooth edges define gaps between electrodes, electrode traces and contact pads and the gaps can be quite small, which provides marked advantages in terms of test accuracy, speed and the number of different functionalities that can be packed into a single biosensor The biosensor production method permits entire electrode patterns to be formed all at one, in nanoseconds and without regard to the complexity of the electrode patterns or the amount of conductive material that must be ablated to form them.

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