Biosensor device and method

G - Physics – 01 – N

Patent

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G01N 27/327 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 27/416 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2251674

A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes an electrode substrate coated with a high-dielectric hydrocarbon-chain monolayer, and having ligands attached to the exposed monolayer surface. Binding of a receptor to the monolayer-bound ligand, and the resultant perturbation of the monolayer structure, causes ion-mediated electron flow across the monolayer. In one embodiment, the monolayers have a coil-coil heterodimer embedded therein, one subunit of which is attached to the substrate, and the second of which carries the ligand at the monolayer surface.

L'invention a trait à un biocapteur permettant de déceler une liaison entre un ligand et un récepteur. Ce biocapteur, qui comporte un substrat, constituant une électrode, revêtu d'une monocouche à chaîne hydrocarbonée à haut pouvoir diélectrique, est pourvu de ligands rattachés à la surface exposée de la monocouche. La liaison d'un récepteur et du ligand fixé à la monocouche ainsi que les perturbations qui en résultent dans la structure de la monocouche entraînent dans celle-ci un flux d'électrons induit par des ions. Dans un mode de réalisation, les monocouches renferment un hétérodimère bispiralé dont une sous-unité est rattachée au substrat et dont l'autre est porteuse du ligand à la surface de la monocouche.

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