Biosensor for detection of and distinguishing between...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/46 (2006.01) C12N 11/12 (2006.01)

Patent

CA 2143618

The invention relates to a biosensor of cholinesterase inhibitors in air, water, foodstuffs, soil, at surfaces and in extracts from samples, comprised of cellulose textile with bioafinity immobilized and stabilized cholinesterase, colour standard and of a carrier from substrate and chromogenic agent and its application to the detection of and distinguishing between cholinesterase in- hibitors. In distinguishing between the inhibitors by a biosensor in case of cholinesterase immobilized at cellulose textile of the biosensor after inhibition by organophosphorous or carbamate inhibitor, hydrolytic activity restores the activity of nucleophilic agents. According to the level of the restored hydrolytic activity of the immobilized cholinesterase in relation to the exposition time to reactivators, e.g. to quaternary aldoximes or water, and according to the activity change of the immobilized cholinesterase exposed in the inhibitor solution and in the inhibitor solution containing ions, e.g. natrium, calcium or ammonium ions, the type of the organophosphorous and carbamate inhibitor is determined.

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