Biosensors for detecting macromolecules and other analytes

Details Biosensors for detecting macromolecules and other analytes Biosensors for detecting macromolecules and other analytes

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A61K 31/7088 (2006.01) A61K 31/713 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2545006

Described are compositions and methods that are useful in the identification and quantification of any polypeptide or macromolecular complex using a set of co-aptamer constructs. Aptamer constructs are constructed that bind to unique epitopes of a polypeptide of macromolecular construct. Those aptamer constructs contain an epitope binding site, a co-aptamer binding site, and a detectable label. In the presence of the cognate polypeptide, analyte- polypeptide complex, or other macromolecular complex, the co-aptamers associate with one another to produce a detectable signal. The co-aptamer constructs may be joined by a linker to produce a bivalent aptamer construct.

L'invention concerne des compositions et des procédés servant à l'identification et à la quantification d'un complexe polypeptidique ou macromoléculaire quelconque, faisant appel à une série de co-aptamères recombinés. Les aptamères recombinés sont conçus de manière à se fixer sur des épitopes spécifiques d'un polypeptide compris dans une structure macromoléculaire. Ces aptamères recombinés contiennent un site de liaison de l'épitope, un site de liaison du co-aptamère et un marqueur détectable. En présence du polypeptide apparenté, d'un complexe analyte-polypeptide, ou d'un autre complexe macromoléculaire, les co-aptamères s'associent mutuellement afin de produire un signal détectable. Les co-aptamères recombinés peuvent être joints par un segment de liaison de manière à former un aptamère recombiné bivalent.

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