Biospecific contrast agents

A - Human Necessities – 61 – B

Patent

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A61B 6/00 (2006.01) A61B 5/00 (2006.01)

Patent

CA 2478083

Methods and apparatuses for detecting a condition of a sample (including, cervical cancers and pre-cancers) through reflectance and/or fluorescence imaging include obtaining a sample and one or more metallic nanoparticles and/or one or more quantum dots (A). The one or more metallic nanoparticles and/or one or more quantum dots are coupled to one or more biomarkers (B) of the sample that are associated with the condition. A reflectance and/or fluorescence image (C) of the sample is then taken. The image(s) exhibit characteristic optical scattering from the one or more metallic nanoparticles and/or characteristic fluorescence excitation from the one or more quantum dots to signal the presence of the one or more biomarkers. In this way, the condition can be readily screened or diagnosed.

L'invention concerne des méthodes et des dispositifs servant à détecter un état d'un échantillon (y compris des cancers et des pré-cancers du col de l'utérus) par imagerie par réflexion et/ou par fluorescence. Les méthodes de l'invention consistent à utiliser un échantillon et une ou plusieurs nanoparticules métalliques et/ou un ou plusieurs points quantiques (A), à relier les nanoparticules métalliques et/ou les points quantiques à un ou plusieurs biomarqueurs (B) de l'échantillon associés à l'état, et à prendre une image (C) par réflexion et/ou par fluorescence de l'échantillon. La ou les images présentent une diffusion optique caractéristique aux nanoparticules et/ou une excitation de fluorescence caractéristique aux points quantiques, indiquant la présence des biomarqueurs. Ainsi, l'état peut être facilement dépisté ou diagnostiqué.

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