C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/18 (2006.01) A01G 33/00 (2006.01) C07K 14/025 (2006.01) C07K 14/475 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2374402
The present invention relates to an economical method for biosynthesis of intended foreign proteins using transformed microalgae, that is to say, it relates to a method of using transformed microalgae as a bioreactor where economical biosynthesis of foreign protein is possible by transforming protoplast of microalgae such as <i>Chlorella ellipsoidea</i> with DNA vector containing intended foreign protein gene and then culturing it in a large scale. In particular, Sh ble gene, which is resistant to phleomycin, is used as a selection marker in the present invention.
L'invention concerne un procédé économique de biosynthèse de protéines étrangères cibles, au moyen de microalgues transformées. L'invention porte notamment sur un procédé d'utilisation de microalgues transformées en tant que bioréacteur, permettant une biosynthèse économique de protéines étrangères par la transformation de protoplaste de microalgues, telles que <i>Chlorella ellipsoidea</i>, à l'aide d'un vecteur d'ADN contenant le gène de la protéine étrangère cible et par sa mise en culture à grande échelle. En particulier, le gène Sh Ble, qui est résistant à la phléomycine, est utilisé comme marqueur de sélection dans le procédé de l'invention.
Choi Tae-Jin
Kim Dae-Hyun
Kim Young-Tae
Algenetech
R. William Wray & Associates
LandOfFree
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