Biosynthetically generated pyrroline-carboxy-lysine and site...

C - Chemistry – Metallurgy – 07 – D

Patent

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C07D 207/20 (2006.01) C07D 209/00 (2006.01) C07K 1/107 (2006.01) C07K 1/13 (2006.01) C07K 2/00 (2006.01) C12P 13/04 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2742043

Disclosed herein is pyrroline-car-boxy-lysine (PCL), a pyrrolysine analogue, which is a natural, bio synthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Image

L'invention concerne une pyrroline-carboxy-lysine (PCL), un analogue de la pyrrolysine qui constitue un acide aminé naturel produit par biosynthèse, et des procédés de production de PCL par biosynthèse. L'invention concerne aussi des protéines, des polypeptides et des peptides intégrant la PCL et des procédés d'intégration de PCL dans des protéines, polypeptides et peptides. L'invention concerne de plus la dérivatisation spécifique de site de protéines, de polypeptides et de peptides intégrant la PCL ou la pyrrolysine. L'invention concerne en outre la réticulation de protéines, de polypeptides et de peptides intégrant la PCL ou la pyrrolysine.

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