Biotechnological method of producing biotin

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/52 (2006.01) C07K 2/00 (2006.01) C12N 1/21 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12P 17/18 (2006.01)

Patent

CA 2145400

The invention pertains to DNA fragments and plasmids comprising the bioB, bioF, bioC, bioD and bioA genes responsible for biosynthesis of biotin, or their functionally equivalent genetic variants and mutants from enteric bacteria, wherein the genes are arranged in a transcription unit. The invention also pertains to microorganisms that contain these DNA fragments and plasmids and a method of producing biotin using these microorganisms.

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