C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) C12N 15/52 (2006.01) C12N 15/67 (2006.01) C12P 17/18 (2006.01)
Patent
CA 2078043
A DNA sequence of a high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided. The invention relates to a DNA sequence of the biotin operon characterized by having at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon which is mutated in comparison with the wild type strain. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin productivity.
Haze Shinitiro
Ifuku Ohji
Kishimoto Jiro
Nakahama Kazuo
Borden Ladner Gervais Llp
Shiseido Company Ltd.
Takeda Chemical Industries Ltd.
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