Biotin-peg-substrate for a lipase assay

C - Chemistry – Metallurgy – 07 – D

Patent

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Details

C07D 495/04 (2006.01) C12Q 1/44 (2006.01) G01N 33/552 (2006.01) G01N 33/573 (2006.01) G01N 33/60 (2006.01)

Patent

CA 2403989

Disclosed is a compound of Formula (I), wherein: L is a linking agent; B is a binding agent; X is an atom or group suitable for attaching L to the glycerol chain; and R is a straight chain saturated or unsaturated alkyl group having from 8 to 30 carbon atoms, substituted with M' or M" wherein at least one of M' and/or M" is a detectable label. The compound can be used as a lipase substrate in a solid phase-based assay system, such as a scintillation proximity assay, to detect lipase enzyme activity.

L'invention concerne un composé représenté par la formule générale (I), dans laquelle L représente un agent de fixation, B représente un agent de liaison, X représente un atome ou un groupe destiné à la fixation de L sur la chaîne glycérol, et R représente un groupe alkyle saturé ou non saturé à chaîne droite, comprenant entre 8 et 30 atomes de carbone, substitué par M' ou M'', M' et/ou M'' représentant un marqueur détectable. Ce composé peut être utilisé en tant que substrat de lipase dans un système de dosage en phase solide, tel qu'un dosage par scintillation de proximité, pour détecter l'activité de l'enzyme lipase.

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