Bispecific antibodies and methods for production thereof

Details Bispecific antibodies and methods for production thereof Bispecific antibodies and methods for production thereof

C - Chemistry, Metallurgy

07

K

C07K 16/46 (2006.01) C07K 16/28 (2006.01)

Patent

CA 2681974

The invention relates to an ex vivo method for the generation of a bispecific antibody, comprising the steps of: a) providing a first antibody having a first binding specificity, wherein said first antibody comprises an IgG4-like CH3 region, b) providing a second antibody having a second binding specificity which differs from said first binding specificity, wherein said second antibody comprises an IgG4-like CH3 region, c) incubating said first and second antibodies together under reducing conditions which allow the cysteines in the core hinge region to undergo disulfidebond isomerization, and d) obtaining a bispecific antibody. The invention furthermore relates to bispecific antibodies obtainable by the method of the invention.

La présente invention concerne un procédé ex vivo permettant de produire un anticorps bispécifique, qui comprend les étapes suivantes : a) utilisation d'un premier anticorps présentant une première spécificité de liaison, ledit premier anticorps comprenant une région CH3 de type IgG4; b) utilisation d'un second anticorps présentant une seconde spécificité de liaison qui diffère de ladite première spécificité de liaison, ledit second anticorps comprenant une région CH3 de type IgG4; c) incubation desdits premier et second anticorps ensemble dans des conditions de réduction qui permettent aux cystéines situées dans la région centrale charnière de subir une isomérisation de liaison disulfure; et d) obtention d'un anticorps bispécifique. L'invention concerne en outre des anticorps bispécifiques qui peuvent être obtenus par le procédé de l'invention.

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