Bleaching compositions comprising multiply-substituted...

C - Chemistry – Metallurgy – 11 – D

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C11D 3/386 (2006.01) A61K 8/66 (2006.01) C11D 3/39 (2006.01) C11D 3/395 (2006.01) C12N 9/54 (2006.01)

Patent

CA 2306894

The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.

La présente invention se rapporte à des compositions de blanchiment comprenant une variante de protéase. Une première composition de blanchiment comprend une variante de protéase renfermant une substitution d'un reste d'acide aminé avec un autre reste d'acide aminé naturel à la position d'un acide aminé correspondant à la position 103 de la subtilisine de Bacillus amyloliquefaciens, en association avec une substitution d'un reste d'acide aminé avec un autre reste d'acide aminé naturel à une ou plusieurs positions d'acide aminé correspondant aux positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 et 275 de la subtilisine de Bacillus amyloliquefaciens; la variante de la protéase précitée comprenant une substitution des restes d'acide aminé aux positions correspondant à la position 103 et 76, avec également une substitution d'un reste d'acide aminé à une ou plusieurs positions d'acide aminé autres que les positions d'acide aminé correspondant aux positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 ou 274 de la subtilisine de Bacillus amyloliquefaciens; un agent de blanchiment, ainsi qu'un ou plusieurs produits de nettoyage complémentaires. Une seconde composition de blanchiment comprend une variante de protéase renfermant une substitution d'un reste d'acide aminé avec un autre reste d'acide aminé naturel à une ou plusieurs positions correspondant aux positions 62, 212, 230, 232, 252 et 257 de la subtilisine de Bacillus amyloliquefaciens; et enfin, un agent de blanchiment et un ou plusieurs produits de nettoyage complémentaires. Cette invention concerne par ailleurs des procédés d'utilisation de ces compositions de blanchiment.

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