Bleaching compositions comprising protease enzymes

Details Bleaching compositions comprising protease enzymes Bleaching compositions comprising protease enzymes

C - Chemistry, Metallurgy

11

D

C11D 3/39 (2006.01) A61K 8/22 (2006.01) A61K 8/23 (2006.01) A61K 8/24 (2006.01) A61K 8/41 (2006.01) A61K 8/42 (2006.01) A61K 8/46 (2006.01) A61K 8/49 (2006.01) A61K 8/66 (2006.01) A61Q 11/00 (2006.01) A61Q 11/02 (2006.01) A61Q 19/10 (2006.01) C11D 3/386 (2006.01)

Patent

CA 2173106

The invention herein provides bleaching compositions comprising a protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position +76 in combination with one or more of the following residues: +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered positions correspond to naturally-occuring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin) and a bleaching agent.

La présente invention concerne des compositions de nettoyage contenant un agent de blanchiment et une enzyme proléolytique qui est une carbonyle hydrolase modifiée ayant une séquence d'acides aminés que l'on ne trouve pas dans la nature et où on a remplacé une pluralité de groupes acides aminés de la carbonyle hydrolase initiale, par d'autres acides aminés. Ce remplacement d'acides aminés de l'enzyme initiale concerne l'acide aminé en position +76, ainsi qu'un ou plusieurs des acides aminés dans les positions suivantes: +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 et/ou +274. Les positions numérotées des acides aminés correspondent à celles des acides aminés de la subtilisine naturelle de <u>Bacillus amyloliquefaciens</u> ou à celles d'autres carbonyle hydrolases ou subtilisines (par exemple subtilisine de <u>Bacillus lentus</u>).

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