Blood cell separation

G - Physics – 01 – N

Patent

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G01N 33/50 (2006.01)

Patent

CA 2660422

There is provided a method of isolating foetal cells from an isolated sample of maternal blood, the method comprising identifying cells having a different expression pattern of at least one foetal marker compared to the expression pattern of the marker in an equivalent maternal cell and selecting the identified cells, characterised in that the foetal marker is selected from: HSP-60, a monoamine oxidase, glutamine synthase, Ara-70, Ara-54, FLJ20202, DCN-I protein, RAB5A, HSP-7C, EFlAl, GRP78, MYL4, DnaJ homolog subfamily B member 14, Vinculin, Desmoplakin, AMMECRl -like protein, Extracellular matrix protein 2 precursor protein, uncharacterised protein Cxorf57, Peroxiredoxin 1, Peroxiredoxin 2. There is also provided a method of cultivating foetal cells and a foetal cell isolation kit.

La présente invention concerne un procédé d'isolement de cellules fAEtales à partir d'un échantillon isolé de sang maternel, le procédé consistant à identifier les cellules dont le profil d'expression d'au moins un marqueur fAEtal est différent du profil d'expression du marqueur dans une cellule maternelle équivalente et à sélectionner les cellules identifiées, caracterisé en ce que le marqueur fAEtal est choisi parmi : HSP-60, une monoamine oxydase, la glutamine synthase, Ara-70, Ara-54, FLJ20202, la protéine DCN-I, RAB5A, HSP-7C, EFlAl, GRP78, MYL4, le membre 14 de la sous-famille B des homologues de DnaJ, la vinculine, la desmoplakine, la protéine AMMECR1, la protéine précurseur de la protéine de la matrice extracellulaire 2, la protéine non caractérisée Cxorf57, la peroxyrédoxine 1, la peroxyrédoxine 2. L'invention concerne également un procédé de mise en culture de cellules fAEtales et un kit pour l'isolement de cellules fAEtales.

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