Blood coagulation promoting product

A - Human Necessities – 61 – K

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167/103.34

A61K 35/16 (2006.01) A61L 2/00 (2006.01) C12N 9/64 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1170183

BLOOD COAGULATION PROMOTING PRODUCT Inventor: Gautam Mitra Abstract of the Disclosure The disclosure pertains to a concentrate for controlling bleeding in hemophilia, free of thrombin, heparin, thromboplastin activity, anticomplement activity, depressor activity, and activated Factor X, and containing coagulation Factors It, VII, IX, and X in non-activated form, having a specific activity of at least about 1.5 Factor IX units per milligram of total protein and a Factor IX: Factor VII ratio of at least about 6:1. In the method of the invention a blood fraction containing coagulation Factors II, VII, IX, and X is applied to an anion exchanger to adsorb the coagulation Factors, which are selectively eluted therefrom using an aqueous sodium chloride-citrate solution of increasing ionic strength. The eluate is treated to reduce its concentration of non-volatile salt and concentrated to yield the above product.

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