Blood constituent count measurement

G - Physics – 01 – N

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G01N 33/49 (2006.01) B01L 3/14 (2006.01) G01N 15/05 (2006.01)

Patent

CA 2022378

INVENTORS: Kristen L. Manion Stephen C. Wardlaw Robert A. Levine TITLE: "BLOOD CONSTITUENT COUNT MEASUREMENT" DOCKET NO.: H-1157 ABSTRACT OF THE DISCLOSURE Blood constituents are separated by centrifugation in a transparent tube which contains a float for physically elongating certain of the constituents. The centrifuged sample in the tube is positioned on a layer measuring device with which red cell and buffy coat constituent band heights can be measured in ambient light. The buffy coat constituent measurements are made under optical magnification. The measuring device has a mathmatically derived red cell layer nomogram printed thereon, and a separate scale for measuring buffy coat constituent bands. Conversion tables are provided for converting the measured buffy coat constituent band lengths into constituent cell counts. The measuring device includes tube-engaging portions for properly positioning the tube during the measurements, and the red cell scale automatically compensates for the presence of the float and densification of red cells needed for proper banding.

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