Blood-fluid composition for cell lysis system

G - Physics – 01 – N

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167/114, 167/45,

G01N 33/53 (2006.01) G01N 33/567 (2006.01)

Patent

CA 1249221

-82- BLOOD-FLUID COMPOSITION FOR CELL LYSIS SYSTEM ABSTRACT A blood fluid composition for use in a complement-mediated cell lysis system. The composition includes a blood fluid, which may be either a serum source of complement, analyte-containing serum, or both, and lipid vesicles capable of reducing the extent of non-specific cell lysis produced when the blood fluid is added to lysable target cells in the system. The vesicles are present in an amount which increases the ratio of ligand-specific to non-specific cell lysis in the system at least 2-fold and preferably 4-fold or more over that achievable in the system in the absence of the vesicles.

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