Blood processing apparatus with controlled cell capture...

B - Operations – Transporting – 01 – D

Patent

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Details

B01D 21/00 (2006.01) B04B 7/00 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2620227

A centrifuge for separating blood components, and methods for controlling the centrifuge. The apparatus has a fluid separation chamber having a first frustro-conical segment and a second frustro-conical segment. The second segment has a taper such that particles are subjected to substantially equal and opposite centripetal and fluid flow forces. A camera observes fluid flow, and a controller controls the flow. White blood cells are selectively captured within the second segment and are periodically flushed out of the fluid separation chamber. The camera is used to determine the quantity of particles captured. A limited quantity of high density particles, such as red blood cells, may be captured within the first segment before capturing relatively low density particles, such as white blood cells, within the second segment.

L'invention porte sur une centrifugeuse permettant de séparer des composants sanguins et sur des procédés de commande de la centrifugeuse. L'appareil possède une chambre de séparation du fluide pourvue d'un premier et d'un second segment frustoconique. Le second segment possède un cône de manière que les particules soient soumises à des forces d'écoulement fluidique et centripète sensiblement égales et opposées. Une caméra observe l'écoulement du fluide et un contrôleur commande cet écoulement. Les globules blancs sont capturés sélectivement dans le second segment et sont évacués périodiquement de la chambre de séparation du fluide. La caméra est utilisée pour déterminer la quantité de particules capturées. Une quantité limitée de particules de haute densité, telles que les globules rouges, peut être capturée dans le premier segment avant la capture des particules de relativement faible densité, telles que les globules blancs, dans le second segment.

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