Blood serum sample prepared for isolation of prions and prpsc

Details Blood serum sample prepared for isolation of prions and prpsc Blood serum sample prepared for isolation of prions and prpsc

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) G01N 1/22 (2006.01) G01N 21/00 (2006.01) G01N 31/00 (2006.01) G01N 33/544 (2006.01) G01N 33/546 (2006.01) G01N 33/551 (2006.01) G01N 33/553 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2362986

A sample is prepared from blood in a manner which makes it possible to further analyze proteins in the sample, e.g. to detect prions in the sample. Blood is extracted, allowed to clot and subjected to separation processing (e.g. centrifugation) to obtain serum. The serum is treated with a complexing agent which binds prions in the sample forming an agent/protein complex which makes it possible to concentrate the complex. Concentration of the complex results in a sample which can be successfully analyzed, e.g. assayed using a range of different types of assay methodologies for detecting prions (see the figure).

On prépare un échantillon de sang d'une manière qui permet de pousser plus loin l'analyse des protéines qu'il contient, par exemple dans le but de détecter des prions. On prélève le sang, on le laisse coaguler et on le soumet à un traitement de séparation (centrifugation par exemple) afin d'obtenir le sérum. On traite ce dernier avec un agent complexant, qui lie les prions contenus dans l'échantillon de façon à former un complexe agent/protéine pouvant être concentré. La concentration du complexe donne un échantillon que l'on peut analyser, par exemple en effectuant des dosages selon différentes méthodologies afin de détecter les prions (voir la figure).

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