Bone marrow-derived neuronal cells

A - Human Necessities – 61 – K

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Details

A61K 35/12 (2006.01) A61K 35/28 (2006.01) A61P 25/28 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2646208

Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC administered into the ventricle did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most orderly integration of BMSC was in the laminar distribution of cerebellar Purkinje cells, where the BMSC-derived cells took on the Purkinje phenotype. The BMSC exhibited site- dependent differentiation and expressed several neuronal markers including neuron- specific nuclear protein, tyrosine hydroxylase and calbindin. Treated BMSC implanted intrastriatally stayed in the cortex and the striatum, produced tyrosine hydroxylase which produces the dopamine needed in Parkinson's disease.

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