Bovine embryo in vitro culture

Details Bovine embryo in vitro culture Bovine embryo in vitro culture

C - Chemistry, Metallurgy

12

N

195/40, 195/46

C12N 5/06 (2006.01) A01K 67/02 (2006.01)

Patent

CA 1319122

ABSTRACT A culture method and system has been realized for in vitro development of bovine embryos from early stage 1- to 2-cell embryos to morula or blastocyst stage. The embryos are placed in a culture system of a buffered balanced salt solution with a carbohydrate energy source medium and bovine oviductal epithelial cells in suspension. Embryo development is enhanced by the addi- tion of bovine or ovine oviductal fluid as well as non-essential amino acids to the system. The method and system can be used to culture genetically manipulated embryos used in transgenic procedures and nuclear transfer.

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