Bowed framed membrane, processes for the preparation...

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2170350

A bowed framed membrane is disclosed for use in automated direct blot electrophoresis and subsequent membrane process steps to develop a chemiluminescent image of the resultant blot of selected fragments and to detect that image. A frame made from a thin rigid plastic plate such as polyethylene is provided with a window. A membrane covers that window and is bonded on two opposing sides only while the frame is held in a bowed condition. The membrane is maintained under tension during processing by the bowing permitting processing at the elevated temperatures used in molecular biological procedures without loss of flatness. Processes for preparing such frames and various utilities are also disclosed.

L'invention décrit une membrane encadrée courbée utilisable dans une électrophorèse par taches directes et les étapes de traitement de la membrane subséquentes visant à produire une image chimioluminescente de la tache de fragments sélectionnés et à détecter cette image. Un cadre fait d'une fine plaque de plastique rigide tel que du polyéthylène est pourvue d'une fenêtre. Une membrane recouvrant cette fenêtre est fixée à deux côtés opposés seulement pendant que le cadre est maintenu courbé. La membrane est maintenue sous tension pendant le traitement de courbure, ce qui lui permet de résister aux températures élevées utilisées dans les procédures de biologie moléculaire sans perdre sa planéité. L'invention décrit également des procédés de fabrication de cadres de ce type et différentes utilisations.

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